مقایسة کارایی بیهوشی با گل میخک، پروپوفول و روش الکتریکی بر مدت زمان و بازگشت از بیهوشی و تأثیر آن بر برخی پاسخ‌های ایمنی و فیزیولوژیک فیل ماهی (Huso huso)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران،کرج، ایران

2 استادیار گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران،کرج، ایران

3 استاد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران،کرج، ایران

4 استاد گروه آموزشی بهداشت و بیماری‌های آبزیان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

10.22059/jfisheries.2023.348600.1342

چکیده

این مطالعه به‌منظور بررسی و مقایسة اثر گل میخک، مادة شیمیایی پروپوفول و روش الکتریکی بر مدت زمان و بازگشت از بیهوشی و تأثیر آن بر برخی پاسخ‌های ایمنی و فیزیولوژیک فیل ماهی (Huso huso) انجام گرفت. بدین‌منظور، 270 قطعه فیل ماهی در 12 عدد ونیرو به‌صورت کاملاً تصادفی ذخیره‌سازی شدند. به‌منظور القای بیهوشی در ماهیان، حمام 300 میلی‌گرم در لیتر پودر گل میخک، 5 میلی‌گرم در لیتر پروپوفول و دستگاه الکتروشوکر در نظر گرفته شد. در هر تیمار بلافاصله پس از رسیدن ماهیان به مرحلة بیهوشی، مدت زمان بیهوشی و زمان بازگشت از آن به‌طور جداگانه ثبت و با هم مقایسه شدند. همچنین برای بررسی اثرات بیهوشی بر ماهی، شاخص‌های خونی (شمارش گلبول قرمز، شمارش گلبول سفید‌، غلظت هموگلوبین و درصد‌‌ هماتوکریت)، شاخص‌های ایمنی (لیزوزیم و فعالیت همولیتیک کمپلمان) و شاخص استرس (گلوگز و کورتیزول) در تیمارها مورد ارزیابی قرار گرفت. در بررسی شاخص های هماتولوژی، بیشترین میزان هموگلوبین در تیمار الکتروشوکر مشاهده شد که تفاوت معنی­داری را با سایر تیمارها داشت و کمترین آن مربوط به تیمار پودر گل میخک بود (0/05>P). بیشترین مقدار هماتوکریت در تیمار الکتروشوکر و کمترین آن در تیمارهای پروپوفول و پودر گل میخک مشاهده شد (0/05>P). نتایج شاخص‌های ایمنی نشان داد بیشترین میزان لیزوزیم و فعالیت کمپلمان ACH50 در تیمار الکتروشوکر و کمترین آن مربوط به تیمار پروپوفول و پودر گل میخک بود (0/05>P). همچنین نتایج شاخص‌های استرس حاکی از آن بود که بیشترین مقدار گلوگز و کورتیزول در تیمار گل میخک و کمترین آن نیز در تیمار الکتروشوکر اندازه ­گیری شد. نتایج این تحقیق با توجه به شاخص‌های ایمنی و استرس وارد شده به ماهی نشان داد که بیهوشی فیل ماهی با روش الکتریکی بهتر از روش گل میخک و پروپوفول بود. از این رو بیهوشی الکتریکی که روشی سریع، ارزان و سازگار با محیط زیست است می‌تواند جایگزین مناسبی برای بیهوشی با مواد شیمیایی باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comparing the efficiency of anesthesia with clove, propofol and electric method on the duration and return from anesthesia and its effect on some immune and physiological responses in the beluga (Huso huso)

نویسندگان [English]

  • Amin Avazeh 1
  • Amirreza Abed Elmdust 2
  • Alireza Mirvaghefi 3
  • Mahdi Soltani 4
1 Ph.D student, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2 Assistant Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
4 Professor, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran
چکیده [English]

This study was conducted in order to investigate and compare the effect of cloves, propofol and electrical methods on the duration and recovery from anesthesia and their effects on some immune and physiological responses in beluga (Huso huso). The 270 beluga fish were randomly stored in 12 tanks. To induce anesthesia, a bath of 300 mg/L of clove powder, 5mg/L of propofol and an electroshock device were used as experimental treatments. In each treatment, immediately after the fish reached the stage of anesthesia, the duration of anesthesia and the recovery time were recorded separately and compared. The treatments were also evaluated for the anesthetic effects on the blood indices (red blood cell count, white blood cell count, hemoglobin concentration and hematocrit percentage), immune parameters (lysozyme and ACH50) and stress parameters (glucose and cortisol). In the examination of hematological indices, the highest level of hemoglobin was obtained in the electroshock treatment, which was significantly different from other treatments, and the lowest was related to clove powder treatment (P<0.05). The highest content of hematocrit was observed in the electroshock treatment and the lowest value was measured in the propofol and clove powder treatments (P<0.05). The results of immune parameters showed that the highest levels of lysozyme and ACH50 were in the electroshock treatment and the lowest values were in the clove powder treatment (P<0.05). Also, the results of stress indicators showed that the highest levels of glucose and cortisol were detected in the clove treatment and the lowest values were in the electroshock treatment. The results of this study revealed that the immune and stress indicators of the fish that underwent electrical anesthesia were better than the clove and propofol methods. Therefore, electrical anesthesia is a fast, cheap, eco-friendly method that can be a potential alternative to chemical anesthesia agents.

کلیدواژه‌ها [English]

  • Electric Anesthesia
  • Propofol
  • Clove
  • Cortisol
  • Lysozyme

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