اثرات نانوپلاستیک پلی استایرن بر شاخص های بافت شناسی کبد و شاخص‌های رشد و بقاء ماهی قزل آلای رنگین‌کمان (Oncorhynchus mykiss)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 فارغ التحصیل کارشناسی ارشد، گروه شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، کرج، ایران

2 دانشیار گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران

3 استادیار گروه شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، کرج، ایران

4 استاد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران

5 دانشیار گروه علوم جانوری، دانشکدة زیست شناسی، دانشگاه تهران، تهران، ایران

6 استاد گروه رزین و مواد افزودنی، پژوهشکدة علوم رنگ و تکنولوژی، تهران، ایران

10.22059/jfisheries.2024.360226.1389

چکیده

علی­رغم توسعة استفاده از نانوپلاستیک پلی­ استایرن در حوزه‌های مختلف نانوفناوری، محیط‌زیست و صنایع و با وجود احتمال راهیابی آن­ ها به بوم­ سازگان ­های آبی، اطلاعات موجود در ارتباط با نانوبوم ­سم ­شناسی آن در آبزیان هنوز بسیار محدود است. در تحقیق حاضر، به منظور سمیت­ سنجی، نانوپلاستیک پلی ­استایرن NPsPS از راه جیرة غذایی، در چهار تیمار با غلظت­های 0 (شاهد)، 1/1، 10 و 100 میلی­ گرم در هر کیلو غذا، به بدن بچه ­ماهیان قزل­ آلای رنگین‌کمان (Oncorhynchus mykiss)، در یک تیمار مزمن 28 روزه و پس از گذشت یک هفته دورة سازگاری، در سه تکرار (در هر تکرار از هر غلظت، 15 عدد بچه ­ماهی)، منتقل شد. نمونه­ گیری از ماهیان بسته به آزمون هدف، 24 ساعت پس از قطع غذا صورت پذیرفت. نتایج نشان دادند که در بالاترین غلظت تغذیه با نانوپلاستیک پلی ­استایرن، نرخ بقا (SR) کاهش معنی­ داری نسبت به سایر تیمارها داشت (0/05>P) ولی سایر شاخص­ های رشد تفاوت آماری معنی­داری نشان ندادند (0/05<P). از منظر آسیب­ شناسی بافتی نانوپلاستیک پلی­استایرن در بافت کبد در تیمار 3 و 4، ماهیان قزل­ آلای رنگین‌کمان جراحاتی نظیر، واکوئولاسیون، نکروز، اتساع سینوزوئیدی و هسته پیکنوتیک مشاهده گردید. براساس نتایج به‌دست آمده، اثرات منفی رهایش نانوپلاستیک پلی ­استایرن بر سلامت و ایمنی در سطح سلولی در بافت­ شناسی کبد در نمونة آبزی مورد مطالعه مشاهده شد. به­ صورت کلی این مطالعه نشان داد، ورود ذرات نانوپلاستیک از طریق لوله گوارش باعث تغییر در پارامتر­های بافت‌شناسی کبد می­ شود. با این وجود مطالعات جامع ­تری در این زمینه مورد نیاز است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of Polystyrene Nanoplastics on Liver Histological Indices and Growth and Survival Indices of Rainbow Trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Melika Mirzaei Nashtoroudi 1
  • Kamran Rezaei Tavabe 2
  • Amirreza Abed Elmdoust 3
  • Gholamreza Rafiee 4
  • Hasan Rahimian 5
  • Behzad Shirkavand Hadavand 6
1 M.Sc. graduate, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2 Associate professor of Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
3 Assistant professor of Department of Fisheries, Faculty of Natural Resources, University of Tehran, Kara
4 Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
5 Professor, Department of Zoology, Faculty of Biology, University of Tehran, Tehran, Iran
6 Professor, Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
چکیده [English]

Despite the development of polystyrene nanoplastics (PSNPs) in different fields, including nanotechnology, environment, industries, and their possible approach to aquatic ecosystems, a few studies have been conducted on nanoecotoxicology in aquatic animals. Therefore, this study was designed and conducted to measure the effect of toxicity of the PSNPs while transferring from the diet to the body of the fish (food concentrations= 0.1, 10, and 100 mg/kg) on juvenile rainbow trout (Oncorhynchus mykiss) in a 28-day chronic treatment and the control group (food concentration = 0 mg/kg) after the 7-day adaptation period, in three repetitions (15 juvenile trout were included in each repetition per concentration). Depending on the target test, fish sampling in the treatment group was taken after 24 h without food. According to the results, the survival rate (SR) (84.44 ± 3.14%) was significantly reduced with the highest dose of PSNPs compared to other treatments (P<0.05). But other growth indices did not show statistically significant difference (P>0.05). From the point of view of tissue pathology of polystyrene nanoplastic in the liver tissue in treatment 3 and 4, injuries such as vacuolation, necrosis, sinusoidal expansion and pyknotic nucleus were observed in rainbow trout. Based on the obtained results, the negative effects of polystyrene nanoplastic release on health and immunity at the cellular level were seen in liver histology in the studied aquatic sample. In general, this study showed that the entry of nanoplastic particles through the digestive tract causes changes in the histological parameters of the liver. However, more comprehensive studies are needed in this field.

کلیدواژه‌ها [English]

  • Polystyrene Nanoplastics
  • survival
  • Liver histology
  • Oncorhynchus mykiss

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شیلات
دوره 77، شماره 1
فروردین 1403
صفحه 25-36

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