بهینه‌سازی استخراج عصارة آنتی‌اکسیدانی از جلبک دریایی خلیج فارس (Chaetomorpha sp) با کمک مایکروویو و اولتراسوند و با استفاده از روش پاسخ سطح

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه فرآوری محصولات شیلاتی، دانشکدة علوم دریایی، دانشگاه تربیت مدرس، نور

2 استاد گروه فرآوری محصولات شیلاتی، دانشکدة علوم دریایی، دانشگاه تربیت مدرس، دانشکدة علوم دریایی تربیت مدرس، شهرستان نور

3 استادیار مؤسسة تحقیقات علوم دامی کشور

4 استادیار گروه شیلات، دانشکدة منابع طبیعی صومعه‌سرا، دانشگاه گیلان

چکیده

جلبک Chaetomorpha sp یکی از جلبک‌های سبز خلیج‌فارس است که می‌تواند به‌منزلة منبعی دریایی حاوی آنتی‌اکسیدان‌های طبیعی از جمله پلی‌فنول‌ها باشد. استخراج و کاربرد ترکیبات آنتی‌اکسیدانی و سایر مواد زیست‌فعال از منابع طبیعی و استفاده از ظرفیت‌های بومی می‌تواند موجب رشد اقتصادی و صنعتی هر منطقه شود. استخراج به کمک مایکروویو و اولتراسوند از جمله روش‌های جدید است و با مزیت‌هایی چون کاهش زمان استخراج و افزایش بهره‌وری انرژی همراه با کاهش تخریب ترکیبات هدف پتانسیل استفاده در آزمایشگاه‌ها را دارد. در این مطالعه برای بهینه‌سازی استخراج ترکیبات آنتی‌اکسیدانی از جلبک C. sp از طراحی مرکب مرکزی در روش مایکروویو شامل 6 تکرار در نقطة مرکزی و 20 دور آزمایشی و در روش اولتراسوند از طرح باکس بنکن شامل 6 تکرار در نقطة مرکزی و 17 دور آزمایشی استفاده شد. خاصیت آنتی‌اکسیدانی با آزمایش‌های میزان فنول کل، قدرت کاهندگی آهن، فعالیت جذب رادیکال آزاد و ظرفیت آنتی‌اکسیدانی‌ کل اندازه‌گیری شد. نتایج نشان داد که بیشترین فعالیت آنتی‌اکسیدانی در شرایط بهینه در روش مایکروویو در قدرت 300، زمان َ8 و غلظت استون به آب 25% و در روش اولتراسوند در زمان 30َ، دمای  30 و غلظت اســتون به آب 0 % به دست آمد و میزان فنول به ترتیب 096/1 و 091/1 میلی‌گرم تانیک‌اسید بر گرم پودر جلبکی بود. مقادیر واقعی آزمایشی و مقادیر پیش‌بینی‌شده با نرم‌افزار نیز دارای روابط منطقی بودند که نشان‌دهندة تناسب مدل‌های به‌کاررفته و نیز سازگاری روش پاسخ سطح برای تعیین شرایط بهینه بوده است. همچنین، جلبک C. sp دارای فعالیت آنتی‌اکسیدانی بود و به‌منزلة منبعی از فنول‌های طبیعی قابلیت استفاده در صنایع غذایی و دارویی را خواهد داشت.
 
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Optimization of microwave and ultrasound-assisted extraction of antioxidant extract from Green marine algae (Chaetomorpha sp) using response surface methodology (RSM)

نویسندگان [English]

  • parva safari 1
  • masud rezaei 2
  • amirreza Shaviklo 3
  • aria babakhani 4
1 MSc student, Department of Seafood processing, Faculty of Marine Science, Tarbiat Modares University,Noor,Mazandaran, Iran
2 Department of Seafood processing, Faculty of Marine Science, Tarbiat Modares University*,Noor, Mazandaran, Iran
3 ssistant Prof., Animal Sciences Reseach Institute of Iran
4 Assistant prof., Faculty of natural resources, Guilan University, Iran
چکیده [English]

Chaetomorpha sp is one of the Persian Gulf green algae which can be a marine source of natural antioxidants such as polyphenols. Industrial and economic development of these materials is influenced by effective extraction and application methods. Microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) are novel techniques which have a potential to apply in laboratory with advantages like reduction in extraction time and target compounds deterioration and enhancement in efficiency. In this study, in MAE a central composite design with six replication in central point and 20 experimental runs and a Box-behnken design with six replications at central point and 17 runs were used in order to optimization of antioxidant components extraction from C.sp. Total phenol content, ferric reducing power, DPPH radical scavenging activity and total antioxidant capacity assays were used to evaluate antioxidant activity of the extracts. The results revealed that the highest antioxidant activity in optimum condition in MAE were in following initial factors: power (300, W), time (8, min) and solvent concentration (25%) and in UAE were: time (8, min), temperature (30, ) and TPC was 1.096 and 1.091 mg tannic acid equivalent/g algae in MAE and UAE, respectively. Moreover, the actual experimental values were adjacent to the corresponding predicted values which demonstrated fitness of the employed models and suitability of RSM in extraction parameters optimization. Furthermore, result revealed the green algae has antioxidant activity and can be used as a resource of natural phenols in food and pharmaceutical industries.
 
 

کلیدواژه‌ها [English]

  • Green algae (Chaetomorpha.sp)
  • phenolic compounds
  • Response Surface Methodology
  • Microwave-assisted extraction
  • Uultrasound-assisted extraction
 

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