اثر تغذیة همزمان پروبیوتیک BioAqua و لسیتین سویا بر عملکرد تولیدمثلی مولدین مادة قزل آلای رنگین‌کمان (Oncorhynchus mykiss)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری گروه شیلات، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

2 استاد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران،کرج ایران

10.22059/jfisheries.2023.95333

چکیده

در تحقیق حاضر اثرات افزودنی پروبیوتیک تجاری BioAqua و لسیتین سویا بر کارایی تولید مثلی (هماوری مطلق و نسبی، تعداد تخمک در گرم، قطر تخمک، درصد لقاح و درصد تخم­های چشم ­زده) در مولدین مادة قزل­ آلای رنگین‌کمان (Oncorhynchus mykiss) بررسی شد. بدین‌منظور 5 جیرة آزمایشی مختلف شامل کنترل، B2L6 (دو گرم بر کیلوگرم پروبیوتیک بایوآکوا و شش درصد لسیتین سویا)، B2L9، B4L6 و B4L9 ساخته شد و مولدین (0/02±2/73 کیلوگرم) به‌مدت دو ماه تغذیه شدند. طی این دوره، تغذیة ماهیان براساس یک درصد وزن بدن آن‌ها یک مرتبه در روز (ساعت 10 صبح) صورت گرفت. بررسی‌ نتایج نشان داد که بالاترین هماوری مطلق و نسبی، درصد لقاح و درصد تخم ­های چشم­ زده در تیمار  B4L9 (به‌ترتیب 76/89±7153/01، 23/77±2604/23، 1±99 درصد و 1/15±97/33درصد) مشاهده شد که اختلاف معنی‌داری با تیمار کنترل (به‌ترتیب 143/09±5304/64، 26/46±1950/05، 1/15±93/33 درصد و 1±91 درصد) داشت (0/05>P). همچنین بیشترین قطر تخمک در تیمارهای B2L9 و B4L9 (به‌ترتیب 0/07±5/01 و 0/04±5/05 میلی­ متر) ثبت شد که اختلاف معنی‌داری با سایر تیمارها داشتند (0/05>P). به‌علاوه، کمترین تعداد تخمک در گرم در تیمار B4L9 (0/3±0/15) به‌دست آمد که اختلاف معنی­داری با تیمار کنترل (0/4±17/2) داشت (0/05>P). نتایج بیانگر آن است که مقادیر هماوری مطلق، هماوری نسبی و تعداد تخمک در گرم در تیمارهای B2L6، B4L6 و B2L9 اختلاف معنی­ داری با یکدیگر ندارند (0/05<P). نتایج پژوهش حاضر نشان داد افزودن همزمان حداقل دو گرم در کیلوگرم پروبیوتیک بایوآکوا به‌همراه حداقل شش درصد لسیتین سویا منجر به بهبود کارایی تولید مثلی مولدین مادة قزل­ آلای رنگین‌کمان خواهد شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of simultaneous feeding of BioAqua probiotics and soy-bean lecithin on reproductive performance of female rainbow trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Faraz Panjvini 1
  • Gholamreza Rafiee 2
1 Ph. D graduate, Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran
2 Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In this study, the effects of different levels of dietary supplementation with probiotic (BioAqua) and soy-bean lecithin on reproductive efficiency (absolute and relative fecundity, number of eggs per gram, egg diameter, percentage of fertilization and percentage of eyed-egg) of female rainbow trout (Oncorhynchus mykiss) were investigated. For this purpose, the broodstock (2.73±0.02 kg) were fed with 5 different experimental diets including control, B2L6 (2 grams/kg of BioAqua probiotics and 6 percent soy lecithin), B2L9, B4L6 and B4L9 with the rate of one percent of their body weight once a day (at 10 A.M) for two months. The highest absolute   relative fecundity, percentage of fertilization, and percentage of eyed-egg were significantly different in B4L9 treatment (7153.01±76.89, 2604.23±23.77, 99±1%, and 97.33±1.15%, respectively) compared to the control treatment (5304.64±143.09, 1950.05±26.46, 93.33±1.15% and 91±1%, respectively; P<0.05). Also, the largest egg diameter was recorded in B2L9 and B4L9 treatments (5.01±0.07 and 5.05±0.04 mm, respectively), which were significantly different from other treatments (P<0.05). Furthermore, the lowest number of eggs per gram was obtained in B4L9 treatment (15±0.3), which had a significant difference with the control treatment (17.2±0.4; P<0.05). The absolute and relative fecundity and number of eggs per gram in B2L6, B4L6 and B2L9 treatments were not significantly different (P>0.05). The result of the current study revealed that simultaneous dietary supplementation of at least two grams per kilogram of BioAqua probiotic along with at least six percent soy-bean lecithin improve the reproductive efficiency in female rainbow trout.

کلیدواژه‌ها [English]

  • Female broodstock
  • Lecithin
  • Probiotics
  • Rainbow trout
  • Reproductive performance
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